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Aptamer-based PDMS–gold nanoparticle composite as a platform for visual detection of biomolecules with silver enhancement by Wei Wang; Wen-Ya Wu; Xiaoqin Zhong; Wei Wang; Qiang Miao; Jun-Jie Zhu is a Engineering article available to read on EtoBox.

What is Aptamer-based PDMS–gold nanoparticle composite as a platform for visual detection of biomolecules with silver enhancement about?

A sensitive colorimetric detection for biomolecules based on aptamer was described. Poly(dimethylsiloxane) (PDMS)-gold nanoparticles (AuNPs) composite film was used as a platform for immobilizing anti-target aptamer. PDMS-AuNPs composite film only covered with aptamer showed high inhibiting ability towards silver reduction, after target molecules were conjugated on the modified surface, the catalytic efficiency of AuNPs for silver reduction was increased. In this system, the darkness density of silver enhancement was applied for target quantitative measurement. Lysozyme and adenosine 5 -triphosphate (ATP) were tested as the models, quantitative measurements with imaging software or semiquantitative measurements with naked eyes were carried out in the range of 1 × 10 -2 -1 g/mL and 1 × 10 -4 -1 × 10 3 g/mL, the volume of reagent using in each assay is 15 L or less. We speculated that aptamer-target conjugates' inhibition ability for AuNPs' catalytic efficiency toward silver reduction might come from charge and spatial effects. This study can offer a completely novel and relatively general approach for colorimetrical aptamer sensors with good analytical properties and potential appli

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Author

Wei Wang; Wen-Ya Wu; Xiaoqin Zhong; Wei Wang; Qiang Miao; Jun-Jie Zhu

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)

Published

2011

Language

EN

Field

Engineering (Physical Sciences)