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Quasi-linear Gradients for Capillary Liquid Chromatography with Mass and Tandem Mass Spectrometry by Jie Zhou; Felicia Rusnak; Tim Colonius; Gary M. Hathaway is a Chemistry article available to read on EtoBox.

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Gradient elution, capillary liquid chromatography mass spectrometry was performed with linear, static gradients constructed by laminar flowing ten, 1.5 mL volume steps of decreasing organic concentration into tubing of small internal diameter. Sample loading, gradient formation, and sample elution were accomplished entirely by means of a commercially available micro-autosampler and single-syringe drive pump. The procedure was simple, fast, stable, and reproducible. Essentially linear gradients were produced without the use of additional valves, mixers, pumps or software. It took less than 10 minutes to form a gradient and less than 30 minutes to construct the set of individual buffer vials. The gradients were shown to be stable to storage. One hour after forming, peak retention times were reproduced to AE0.5%. Long-term retention time reproducibility was found to vary by AE2%. Chromatographic resolution was comparable or superior to that obtained by gradient elution with conventional dynamic mixing and split flow. The procedure was adapted with a 'peak parking' method which extended the time for generating peptide fragmentation data up to 10 minutes per peptide with the triple quad

Who reads Quasi-linear Gradients for Capillary Liquid Chromatography with Mass and Tandem Mass Spectrometry?

Author

Jie Zhou; Felicia Rusnak; Tim Colonius; Gary M. Hathaway

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2000

Language

EN

Field

Chemistry (Physical Sciences)