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ERK 1/2 activation in enhanced osteogenesis of human mesenchymal stem cells in poly(lactic-glycolic acid) by cyclic hydrostatic pressure by Su-Hyang Kim; Yon Rak Choi; Min Sung Park; Jung Woog Shin; Ki Dong Park; Sung-Jae Kim; Jin Woo Lee is a Engineering article available to read on EtoBox.

What is ERK 1/2 activation in enhanced osteogenesis of human mesenchymal stem cells in poly(lactic-glycolic acid) by cyclic hydrostatic pressure about?

## Abstract The aim of this study was to identify the signal transduction pathways and mechano‐transducers that play critical roles in the processes induced by changes in cyclic hydrostatic pressure and fluid shear in 3‐dimensional (3D) culture systems. Mesenchymal stem cells were loaded into a polymeric scaffold and divided into three groups according to the stress treatment: static, fluid shear, and hydrostatic pressure with fluid shear. Cells were exposed daily to a hydrostatic pressure of 0.2 MPa for 1 min followed by 14 min rest with fluid flow at 30 rpm. Protein extracts were analyzed by Western blot for extracellular signal‐regulated kinase 1/2 (ERK1/2). The complexes were cultured under the mechanical stimuli for 21 days with or without phospho‐ERK1/2 inhibitor (U0126) and evaluated by RT‐PCR, calcium contents, and immunohistochemistry. Under conditions of mechanical stimulation, the activation of ERK1/2 was sustained or increased with time. U0126 suppressed mechanical stimuli‐induced expression of osteocalcin. In addition, calcium contents and the degrees of osteocalcin and osteopontin staining were decreased by this inhibitor. These results demonstrate that mechanical sti

Who reads ERK 1/2 activation in enhanced osteogenesis of human mesenchymal stem cells in poly(lactic-glycolic acid) by cyclic hydrostatic pressure?

Author

Su-Hyang Kim; Yon Rak Choi; Min Sung Park; Jung Woog Shin; Ki Dong Park; Sung-Jae Kim; Jin Woo Lee

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2006

Language

EN

Field

Engineering (Physical Sciences)