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Similar UV responses are seen in a skin organ culture as in human skin in vivo by Helena Bäckvall; Cecilia Wassberg; Berit Berne; Fredrik Ponten is a Medicine article available to read on EtoBox.

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**Abstract:** Ultraviolet radiation (UVR) plays an important role in the development of non‐melanoma skin cancer. Most tumors develop in chronically sun‐exposed skin, most often in cosmetically sensitive locations, where __in vivo__ experiments may be difficult to perform. In this study, we describe a skin organ culture model with preserved normal morphology and intact response to UVR. Skin explants from chronically sun‐exposed and non‐sun‐exposed skin were irradiated with artificial UVA+UVB with and without topical sunscreen. UV‐induced DNA damage, epidermal p53 response and repair kinetics were analyzed using immunohistochemistry. Four hours after UV‐irradiation epidermal keratinocytes showed a strong immunoreactivity for thymine‐dimers. Gradual repair during an incubation time resulted in few residual thymine‐dimers after 48 h. Repair appeared to be more efficient in chronically sun‐exposed skin compared with non‐sun‐exposed skin. There was also an accumulation of p53 protein in epidermal keratinocytes, peaking at 4–24 h after irradiation. Large interindividual differences with respect to formation and repair of thymine‐dimers as well as induction and duration of the p53 respons

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Author

Helena Bäckvall; Cecilia Wassberg; Berit Berne; Fredrik Ponten

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0906-6705)

Published

2002

Language

EN

Field

Medicine (Health Sciences)