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Connective Tissue Growth Factor Promoter Activity in Normal and Wounded Skin by Mohit Kapoor; Shangxi Liu; Kun Huh; Sunil Parapuram; Laura Kennedy; Andrew Leask is a Medicine article available to read on EtoBox.

What is Connective Tissue Growth Factor Promoter Activity in Normal and Wounded Skin about?

In skin, connective tissue growth factor (CTGF/CCN2) is induced during tissue repair. However, what the exact cell types are that express CTGF in normal and wounded skin remain controversial. In this report, we use transgenic knock-in mice in which the Pacific jellyfish __Aequorea victoria__ enhanced green fluorescent protein (E-GFP) gene has been inserted between the endogenous CTGF promoter and gene. Unwounded (day 0) and wounded (days 3 and 7) skin was examined for GFP to detect cells in which the CTGF promoter was active, α-smooth muscle actin (α-SMA) to detect myofibroblasts, and NG2 expression to detect pericytes. In unwounded mice, CTGF expression was absent in epidermis and was present in a few cells in the dermis. Upon wounding, CTGF expression was induced in the dermis. Double immunolabeling revealed that CTGF-expressing cells also expressed α-SMA, indicating the CTGF was expressed in myofibroblasts. A subset (~30%) of myofibroblasts were also NG2 positive, indicating that pericytes significantly contributed to the number of myofibroblasts in the wound. Pericytes also expressed CTGF. Collectively, these results indicate that CTGF expression in skin correlates with myofibr

Who reads Connective Tissue Growth Factor Promoter Activity in Normal and Wounded Skin?

Author

Mohit Kapoor; Shangxi Liu; Kun Huh; Sunil Parapuram; Laura Kennedy; Andrew Leask

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2008-; Springer Science and Business Media LLC (ISSN 1755-1536)

Published

2008

Language

EN

Field

Medicine (Health Sciences)