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Antimicrobial Protein Produced by Vaginal Lactobacillus acidophilus that inhibits Gardnerella vaginalis by Alla A. Aroutcheva; Jose A. Simoes; Sebastian Faro is a Medicine article available to read on EtoBox.

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__Objective:__ To isolate bacteriocin from a vaginal strain of __Lactobacillus acidophilus__. __Methods: L. acidophilus__ 160 was grown on two media. The first was MRS broth for 18 hours; the cells were harvested, washed, and placed into a chemically defined medium. The second medium resembled vaginal fluid minus protein. Bacteriocin was precipitated from both media using ammonium sulfate. The growth-inhibiting activity of bacteriocin was determined by a bioassay using nine different isolates of __Gardnerella vaginalis__. __Results:__ MRS broth is not a suitable medium for extracting bacteriocin, because it binds with Tween 80. Bacteriocin was isolated, without contaminating constituents, from chemically defined medium and identified as a single band by electrophoresis. Bacteriocin has a molecular weight of 3.8 kDa. All nine isolates of __Gardnerella__ were inhibited by the bacteriocin isolated from __L. acidophilus__ 160. __Conclusions:__ Bacteriocin produced by __L. acidophilus__ 160 was isolated from the chemically defined medium (starvation medium) in a partially pure form. __L. acidophilus__ 160 bacteriocin inhibited growth of all nine isolates of __Gardnerella vaginalis__.

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Author

Alla A. Aroutcheva; Jose A. Simoes; Sebastian Faro

Publisher

Hindawi Publishing Corporation

Published

2001

Language

EN

Field

Medicine (Health Sciences)