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Structure of the Calx-β domain of the integrin β4 subunit: insights into function and cation-independent stability by Noelia Alonso-García; Alvaro Inglés-Prieto; Arnoud Sonnenberg; Jose M. de Pereda is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Structure of the Calx-β domain of the integrin β4 subunit: insights into function and cation-independent stability about?
The integrin alpha6beta4 is a receptor for laminins and provides stable adhesion of epithelial cells to the basement membranes. In addition, alpha6beta4 is important for keratinocyte migration during wound healing and favours the invasion of carcinomas into surrounding tissue. The cytoplasmic domain of the beta4 subunit is responsible for most of the intracellular interactions of the integrin; it contains four fibronectin type III domains and a Calx-beta motif. The crystal structure of the Calx-beta domain of beta4 was determined to 1.48 A resolution. The structure does not contain cations and biophysical data support the supposition that the Calx-beta domain of beta4 does not bind calcium. Comparison of the Calx-beta domain of beta4 with the calcium-binding domains of Na(+)/Ca(2+)-exchanger 1 reveals that in beta4 Arg1003 occupies a position equivalent to that of the calcium ions in the Na(+)/Ca(2+)-exchanger. By combining mutagenesis and thermally induced unfolding, it is shown that Arg1003 contributes to the stability of the Calx-beta domain. The structure of the Calx-beta domain is discussed in the context of the function and intracellular interactions of the integrin beta4 sub
Who reads Structure of the Calx-β domain of the integrin β4 subunit: insights into function and cation-independent stability?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Noelia Alonso-García; Alvaro Inglés-Prieto; Arnoud Sonnenberg; Jose M. de Pereda
- Publisher
- International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)