About this Engineering article
Universal Detection for Capillary Electrophoresis-using Micro-interferometric Backscatter Detection by Kelly A. Swinney; Darryl J. Bornhop is a Engineering article available to read on EtoBox.
We describe a method for determining subpicomole quantities of analyte in capillary electrophoresis by probing fluid bulk properties with laser interferometric backscatter. In micro-interferometric backscatter detection Ž . MIBD , an unfocused beam from a He-Ne laser impinges onto unmodified fused silica capillary tube and subsequent backscattered laser light is analyzed. Positional Ž . changes of interference pattern extrema backscattered light are directly related to Ž . refractive index RI , n, of fluid in the tube, and allow for determination of ⌬ n at the 10 y7 level. MIBD was interfaced to commercial CE instrument allowing for simultaneous RIr UV-Vis detection. Utility of MIBD detector for capillary electrophoresis was shown by detecting caffeine in beverages. Analysis was performed in 100-m capillary, and direct comparison of RI detector's performance with Ž . UV-Vis absorbance detector 211 nm was possible. In system without active Ž . y6 thermal control, RI detection limit 3 for caffeine was 2.33)10 M, whereas detection limit of the commercial UV-Vis detector was 1.16)10 y6 M. At detection limit of 2.33 M, MIBD is capable of performing universal detection on just 2.02)10 y1
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- Author
- Kelly A. Swinney; Darryl J. Bornhop
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1040-7685)
- Published
- 1999
- Language
- EN
- Field
- Engineering (Physical Sciences)