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Can I read Measurement of the Release of Adenine Nucleotides During Platelet Aggregation by Small-bore-column Isocratic High-performance Liquid Chromatography on EtoBox?
Measurement of the Release of Adenine Nucleotides During Platelet Aggregation by Small-bore-column Isocratic High-performance Liquid Chromatography by F. Bodola; C.R. Benedict is a Chemistry article available to read on EtoBox.
What is Measurement of the Release of Adenine Nucleotides During Platelet Aggregation by Small-bore-column Isocratic High-performance Liquid Chromatography about?
This paper describes a simple and sensitive method for measuring adenosine, adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate in plasma and tissues. This method involves sample preparation by perchloric acid extraction, followed by high-performance liquid chromatography on a small-bore column with UV detection at 254 nm. The minimum detectable amounts were adenosine, 0.60 pmol; adenosine monophosphate, 0.90 pmol; adenosine diphosphate, 1.5 pmol; and adenosine triphosphate, 2.5 pmol. The interassay coefficient of variation for the measurement of these compounds was less than 4%. The influence of varying the pH and the concentration of phosphate, acetonitrile, and ion-pairing reagent are described. This method can be used to measure adenine nucleotide release during platelet aggregation.
Who reads Measurement of the Release of Adenine Nucleotides During Platelet Aggregation by Small-bore-column Isocratic High-performance Liquid Chromatography?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- F. Bodola; C.R. Benedict
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1988
- Language
- EN
- Field
- Chemistry (Physical Sciences)