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Engineering the Staple Oil Crop Brassica napus Enriched with α-Linolenic Acid Using the Perilla FAD2 – FAD3 Fusion Gene by Yu-Fei Xue; Chun Fu; Cheng-Yan Chai; Fei-Fei Liao; Bao-Jun Chen; Song-Zhen Wei; Rui Wang; Huan Gao; Teng-Teng Fan; You-Rong Chai is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Engineering the Staple Oil Crop Brassica napus Enriched with α-Linolenic Acid Using the Perilla FAD2 – FAD3 Fusion Gene about?

Modern people generally suffer from α-linolenic acid (ALA) deficiency, since most staple food oils are low in ALA content. Thus, the enhancement of ALA in staple oil crops is of importance. In this study, the FAD2 and FAD3 coding regions from the ALA-king species Perilla frutescens were fused using a newly designed double linker LP4-2A, driven by a seed-specific promoter P NAP , and engineered into a rapeseed elite cultivar ZS10 with canola quality background. The mean ALA content in the seed oil of P NAP :Pf FAD2−Pf FAD3 (N23) T5 lines was 3.34-fold that of the control (32.08 vs 9.59%), with the best line being up to 37.47%. There are no significant side effects of the engineered constructs on the background traits including oil content. In fatty acid biosynthesis pathways, the expression levels of structural genes as well as regulatory genes were significantly upregulated in N23 lines. On the other hand, the expression levels of genes encoding the positive regulators of flavonoid-proanthocyanidin biosynthesis but negative regulators of oil accumulation were significantly downregulated. Surprisingly, the ALA level in Pf FAD2−Pf FAD3 transgenic rapeseed lines driven by the constitu

Who reads Engineering the Staple Oil Crop Brassica napus Enriched with α-Linolenic Acid Using the Perilla FAD2 – FAD3 Fusion Gene?

Author

Yu-Fei Xue; Chun Fu; Cheng-Yan Chai; Fei-Fei Liao; Bao-Jun Chen; Song-Zhen Wei; Rui Wang; Huan Gao; Teng-Teng Fan; You-Rong Chai

Publisher

American Chemical Society (ACS)

Published

2023

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)