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Fed-batch Cultivation of Animal Cells Using Different Medium Design Concepts and Feeding Strategies by Liangzhi Xie; Daniel I.C. Wang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract In animal cell cultivation, cell density and product concentration are often low due to the accumulation of toxic end‐products such as ammonia and lactate and/or the depletion of essential nutrients. A hybridoma cell line (CRL‐1606) was cultivated in T‐flasks using a newly devised medium feeding strategy. The goals were to decrease ammonia and lactate formation by the design of an initial medium which would provide a starting environment to achieve optimal cell growth. This was followed by using a stoichiometric equation governing animal cell growth and then designing a supplemental medium for feeding strategy used to control the nutritional environment. The relationship between the stoichiometric demands for glutamine and nonessential amino acids was also studied. Through stoichiometric feeding, nutrient concentrations were controlled reasonably well. Consequently, the specific production rate of lactate was decreased by fourfold compared with conventional fed‐batch culture and by 26‐fold compared with conventional batch culture. The specific production rate of ammonia was decreased by tenfold compared with conventional fed‐batch culture and by 50‐fold compared with co

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Author

Liangzhi Xie; Daniel I.C. Wang

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)