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Crystallization Of Nucleic Acids And Proteins A Practical Approach Second Edition by Arnaud Ducruix, Richard Giegé is a book available to read on EtoBox.

What is Crystallization Of Nucleic Acids And Proteins A Practical Approach Second Edition about?

Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucliec Acids and Proteins: A Practical Approach continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two- dimensional electron crystallography of soluble proteins on planar lipid films. As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminary characterization

Author

Arnaud Ducruix, Richard Giegé

Publisher

Oxford University Press, U.S.A.

Published

1999

Language

EN

ISBN

9780585484129

Subjects

Science, Technology, Chemistry