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Can I read Single-cell RNA-sequencing of stria vascularis cells in the adult Slc26a4 ^-/-^ mouse on EtoBox?
Single-cell RNA-sequencing of stria vascularis cells in the adult Slc26a4 ^-/-^ mouse by Jin-Young Koh; Corentin Affortit; Paul T. Ranum; Cody West; William D. Walls; Hidekane Yoshimura; Jian Q. Shao; Brian Mostaert; Richard J.H. Smith is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Single-cell RNA-sequencing of stria vascularis cells in the adult Slc26a4 ^-/-^ mouse about?
## Background The primary pathological alterations of Pendred syndrome are endolymphatic pH acidification and luminal enlargement of the inner ear. However, the molecular contributions of specific cell types remain poorly characterized. Therefore, we aimed to identify pH regulators in pendrin-expressing cells that may contribute to the homeostasis of endolymph pH and define the cellular pathogenic mechanisms that contribute to the dysregulation of cochlear endolymph pH in __Slc26a4__^−/−^ mice. ## Methods We used single-cell RNA sequencing to identify both __Slc26a4__-expressing cells and __Kcnj10__-expressing cells in wild-type (WT, __Slc26a4__^+/+^) and __Slc26a4__^−/−^ mice. Bioinformatic analysis of expression data confirmed marker genes defining the different cell types of the stria vascularis. In addition, specific findings were confirmed at the protein level by immunofluorescence. ## Results We found that spindle cells, which express pendrin, contain extrinsic cellular components, a factor that enables cell-to-cell communication. In addition, the gene expression profile informed the pH of the spindle cells. Compared to WT, the transcriptional profiles in __Slc26a4__^−/−^ mic
Who reads Single-cell RNA-sequencing of stria vascularis cells in the adult Slc26a4 ^-/-^ mouse?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jin-Young Koh; Corentin Affortit; Paul T. Ranum; Cody West; William D. Walls; Hidekane Yoshimura; Jian Q. Shao; Brian Mostaert; Richard J.H. Smith
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)