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Analysis of the Microheterogeneity of the IgA1 Hinge Glycopeptide Having Multiple O-Linked Oligosaccharides by Capillary Electrophoresis by Hitoo Iwase; Atsushi Tanaka; Yoshiyuki Hiki; Tohru Kokubo; Takashi Sano; Ikuko Ishii-Karakasa; Kunio Hisatani; Yutaka Kobayashi; Kyoko Hotta is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Analysis of the Microheterogeneity of the IgA1 Hinge Glycopeptide Having Multiple O-Linked Oligosaccharides by Capillary Electrophoresis about?
It was found that the self-aggregation of IgA1 was closely connected with the glycoform of a mucintype sugar chain on its hinge portion. In this report, normal human serum IgA1 was separated into two subfractions by a jacalin column. The elution condition, 25 mM galactose, used here was similar to that reported for the glycoprotein with a single mucintype sugar chain per molecule. The IgA1 eluted under this condition was substantially the monomeric form. In contrast, the remaining IgA1 eluted from the column with 0.8 M galactose was substantially the aggregated form. An analytical method for the microheterogeneity of the IgA1 hinge glycopeptide (HGP33) was developed to determine the difference between these IgA1 fractions by capillary electrophoresis (CE). Native HGP33 from both IgA1 fractions was separated into peaks 1-11, depending on their glycoforms. Because the sialic acid-rich component migrated slowly on CE, the 25 mM fraction was abundant in the sialic acid-rich components (peaks 7-11), but the 0.8 M fraction was abundant in the sialic acid-poor components (peaks 1-4). Comparison of the number of sugar chains per hinge peptide indicated that the 25 mM fraction was relativel
Who reads Analysis of the Microheterogeneity of the IgA1 Hinge Glycopeptide Having Multiple O-Linked Oligosaccharides by Capillary Electrophoresis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hitoo Iwase; Atsushi Tanaka; Yoshiyuki Hiki; Tohru Kokubo; Takashi Sano; Ikuko Ishii-Karakasa; Kunio Hisatani; Yutaka Kobayashi; Kyoko Hotta
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 2001
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)