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Null mutation inshaking-B eliminates electrical, but not chemical, synapses in theDrosophila giant fiber system: A structural study by Blagburn, Jonathan M.; Alexopoulos, Haralambos; Davies, Jane A.; Bacon, Jonathan P. is a Neuroscience article available to read on EtoBox.

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Mutations in the Drosophila shaking-B gene perturb synaptic transmission and dye coupling in the giant fiber escape system. The GAL4 upstream activation sequence system was used to express a neuronal-synaptobrevin-green fluorescent protein (nsyb-GFP) construct in the giant fibers (GFs); nsyb-GFP was localized where the GFs contact the peripherally synapsing interneurons (PSIs) and the tergotrochanteral motorneurons (TTMns). Antibody to Shaking-B protein stained plaquelike structures in the same regions of the GFs, although not all plaques colocalized with nsyb-GFP. Electron microscopy showed that the GF-TTMn and GF-PSI contacts contained many chemical synaptic release sites. These sites were interposed with extensive regions of close membrane apposition (3.25 nm Ϯ 0.12 separation), with faint cross striations and a single-layered array of 41-nm vesicles on the GF side of the apposition. These contacts appeared similar to rectifying electrical synapses in the crayfish and were eliminated in shaking-B 2 mutants. At mutant GF-TTMn and GF-PSI contacts, chemical synapses and small regions of close membrane apposition, more similar to vertebrate gap junctions, were not affected. Gap junc

Who reads Null mutation inshaking-B eliminates electrical, but not chemical, synapses in theDrosophila giant fiber system: A structural study?

Author

Blagburn, Jonathan M.; Alexopoulos, Haralambos; Davies, Jane A.; Bacon, Jonathan P.

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0021-9967)

Published

1999

Language

EN

Field

Neuroscience (Life Sciences)