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This document provides instructions for making ATCC Medium 2436, which is used to culture the bacteria Acidothiobacillus ferrooxidans. The medium contains (NH4)2SO4, K2HPO4, MgSO4.7H2O, KCl, and Ca(NO3)2 in solution 1, along with FeSO4.7H2O in solution 2, with the solutions adjusted to pH 5.5 and 1.4, respectively, before being combined and distributed into sterile tubes for long term dark storage.

Author

Claudio Quiñones Cerna

Language

EN