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Raman Spectroscopy of Blood Samples for Forensic Applications by Samantha Boyd; Massimo F. Bertino; Sarah J. Seashols is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Raman Spectroscopy of Blood Samples for Forensic Applications about?

We investigated Raman scattering from human blood as a function of parameters that are relevant for forensic field analysis, such as substrate, sample dilution, individual from which the sample originates, and age of the sample. Peaks characteristic of blood components and in particular the hemoglobin peaks were routinely detected when blood was deposited on substrates that are not strongly luminescent, such as plastic, metal utensils and dry wall. Raman scattering from blood proved quite sensitive and blood samples with a dilution up to 1:250 could be measured for an excitation power of ∼2 mW measured at the sample plane. The sensitivity of Raman scattering to diluted blood allowed measurement using blood reconstituted from fabric substrates, thereby alleviating issues related to luminescence and scattering from the substrate. The dependence of Raman scattering on sample age and individual was also investigated. We found that the relative intensities of scattering peaks depended on sample age and history. For example, the relative intensity of oxyhemoglobin peaks decreases after blood has dried. Fresh blood drawn directly from a donor without intermediate storage exhibits also sca

Who reads Raman Spectroscopy of Blood Samples for Forensic Applications?

Author

Samantha Boyd; Massimo F. Bertino; Sarah J. Seashols

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0379-0738)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)