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Copper stress proteomics highlights local adaptation of two strains of the model brown alga Ectocarpus siliculosus by Andrés Ritter; Martin Ubertini; Sarah Romac; Fanny Gaillard; Ludovic Delage; Aaron Mann; J. Mark Cock; Thierry Tonon; Juan A. Correa; Philippe Potin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Copper stress proteomics highlights local adaptation of two strains of the model brown alga Ectocarpus siliculosus about?
## Abstract __Ectocarpus siliculosus__ is a cosmopolitan brown alga with capacity to thrive in copper enriched environments. Analysis of copper toxicity was conducted in two strains of __E. siliculosus__ isolated from (i) an uncontaminated coast in southern Peru (Es32) and (ii) a copper polluted rocky beach in northern Chile (Es524). Es32 was more sensitive than Es524, with toxicity detected at 50 μg/L Cu, whereas Es524 displayed negative effects only when exposed to 250 μg/L Cu. Differential soluble proteome profiling for each strain exposed to sub‐lethal copper levels allowed to identify the induction of proteins related to processes such as energy production, glutathione metabolism as well as accumulation of HSPs. In addition, the inter‐strain comparison of stress‐related proteomes led to identify features related to copper tolerance in Es524, such as striking expression of a PSII Mn‐stabilizing protein and a Fucoxanthine chlorophyll a–c binding protein. Es524 also expressed specific stress‐related enzymes such as RNA helicases from the DEAD box families and a vanadium‐dependent bromoperoxidase. These observations were supported by RT‐qPCR for some of the identified genes and an
Who reads Copper stress proteomics highlights local adaptation of two strains of the model brown alga Ectocarpus siliculosus?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Andrés Ritter; Martin Ubertini; Sarah Romac; Fanny Gaillard; Ludovic Delage; Aaron Mann; J. Mark Cock; Thierry Tonon; Juan A. Correa; Philippe Potin
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)