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Methylobacterium sp. isolated from a Finnish paper machine produces highly pyruvated galactan exopolysaccharide by René Verhoef; Pieter de Waard; Henk A. Schols; Matti Siika-aho; Alphons G.J. Voragen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The slime-forming bacterium Methylobacterium sp. was isolated from a Finnish paper machine and its exopolysaccharide (EPS) was produced on laboratory scale. Sugar compositional analysis revealed a 100% galactan (EPS). However, FT-IR showed a very strong peak at 1611 cm (1 showing the presence of pyruvate. Analysis of the pyruvate content revealed that, based on the sugar composition, the EPS consists of a trisaccharide repeating unit consisting of D-galactopyranose and [4,6-O -(1-carboxyethylidene)]-D-galactopyranose with a molar ratio of 1:2, respectively. Both linkage analysis and 2D homo-and heteronuclear 1 H and 13 C NMR spectroscopy revealed the following repeating unit: ]-a-D-Galp-(10/3)-a-D-Galp-(1 0/. By enrichment cultures from various ground and compost heap samples a polysaccharidedegrading culture was obtained that produced an endo acting enzyme able to degrade the EPS described. The enzyme hydrolysed the EPS to a large extent, releasing oligomers that mainly consisted out of two repeating units.

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Author

René Verhoef; Pieter de Waard; Henk A. Schols; Matti Siika-aho; Alphons G.J. Voragen

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)