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Can I read Targeted Electroporation of Defined Lateral Ventricular Walls: a Novel and Rapid Method to Study Fate Specification During Postnatal Forebrain Neurogenesis on EtoBox?
Targeted Electroporation of Defined Lateral Ventricular Walls: a Novel and Rapid Method to Study Fate Specification During Postnatal Forebrain Neurogenesis by María E Fernández; Simona Croce; Camille Boutin; Harold Cremer; Olivier Raineteau is a Neuroscience article available to read on EtoBox.
What is Targeted Electroporation of Defined Lateral Ventricular Walls: a Novel and Rapid Method to Study Fate Specification During Postnatal Forebrain Neurogenesis about?
## Background Postnatal olfactory bulb (OB) neurogenesis involves the generation of granule and periglomerular cells by neural stem cells (NSCs) located in the walls of the lateral ventricle (LV). Recent studies show that NSCs located in different regions of the LV give rise to different types of OB neurons. However, the molecular mechanisms governing neuronal specification remain largely unknown and new methods to approach these questions are needed. ## Results In this study, we refine electroporation of the postnatal forebrain as a technique to perform precise and accurate delivery of transgenes to NSCs located in distinct walls of the LV in the mouse. Using this method, we confirm and expand previous studies showing that NSCs in distinct walls of the LV produce neurons that invade different layers of the OB. Fate mapping of the progeny of radial glial cells located in these distinct LV walls reveals their specification into defined subtypes of granule and periglomerular neurons. ## Conclusions Our results provide a baseline with which future studies aiming at investigating the role of factors in postnatal forebrain neuronal specification can be compared. Targeted electroporation
Who reads Targeted Electroporation of Defined Lateral Ventricular Walls: a Novel and Rapid Method to Study Fate Specification During Postnatal Forebrain Neurogenesis?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- María E Fernández; Simona Croce; Camille Boutin; Harold Cremer; Olivier Raineteau
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2006-; Springer Science and Business Media LLC (ISSN 1749-8104)
- Published
- 2011
- Language
- EN
- Field
- Neuroscience (Life Sciences)