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Subcellular distribution of simian virus 40 T antigen species in various cell lines: The 56K protein by Samuel W. Luborsky; K. Chandrasekaran is a Medicine article available to read on EtoBox.

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## Abstract The subcellular distribution of SV40 anti‐T serumspecific species was examined in SV40‐transformed, Tantigen‐positive tissue culture cell lines of rat and of AL/N and BALB/c mouse origin. Cells were labelled with [^35^S]methionine. The cytoplasm, nuclear and membrane fractions were obtained, and their radioimmunoprecipitates analyzed by gel electrophoresis. Tests were performed to determine the purity of these subcellular fractions, and negligible cross‐contamination was found. The cytoplasm fractions lacked detectable anti‐T serum reactivity. Large amounts of both large T antigen and a 56K protein were always present together both in the nuclear fractions and, in a somewhat lesser amount, in the plasma membrane fractions of all cell lines examined. Analysis of density gradient sedimentation profiles of the immunoprecipitates of whole‐cell extracts indicated these species were associated in some fashion, probably with each other. The activity of the 56K protein may be associated with its presence on the cell surface where, either alone or acting together with the large T antigen, it might provide the surface activity responsible for tumor‐specific surface and/or tranepl

Who reads Subcellular distribution of simian virus 40 T antigen species in various cell lines: The 56K protein?

Author

Samuel W. Luborsky; K. Chandrasekaran

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1980

Language

EN

Field

Medicine (Health Sciences)