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Drug Permeability Profiling Using the Novel Permeapad® 96-Well Plate by Ann-Christin Jacobsen; Sune Nielsen; Martin Brandl; Annette Bauer-Brandl is a Pharmacology, Toxicology and Pharmaceutics article available to read on EtoBox.

What is Drug Permeability Profiling Using the Novel Permeapad® 96-Well Plate about?

## Purpose: Here, first experiences with a prototype tool for high throughput (passive) permeability profiling, a 96-well plate comprising the permeapad® membrane, are reported. the permeabilities of a set of drugs were determined and compared to published measures of oral absorption, such as human fraction absorbed (f) and in vitro permeability values obtained using other tools. ## Methods: The tool consists of a 96-well bottom and screen plate with the artificial, phospholipid-based barrier (permeapad®) mounted between the plates' lower and upper compartments. the permeability of 14 model compounds including high- and low-absorption drugs, cationic, anionic, zwitterionic and neutral molecules, was determined by quantifying the compounds' transport over time, deriving the steady-state flux from the linear part of the cumulative curves and calculating the apparent permeability (p). the membrane structure was investigated in a high-resolution digital light microscope. ## Results: The permeapad® 96-well plate was found suited to distinguish high and low absorption drugs and yielded a hyperbolic correlation to f. the p values obtained were congruent with those determined with in-house

Who reads Drug Permeability Profiling Using the Novel Permeapad® 96-Well Plate?

Author

Ann-Christin Jacobsen; Sune Nielsen; Martin Brandl; Annette Bauer-Brandl

Publisher

Springer US; Springer-Verlag; Kluwer Academic/Plenum Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0724-8741)

Published

2020

Language

EN

Field

Pharmacology, Toxicology and Pharmaceutics (Life Sciences)