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Can I read HER 2/neu expression and gene amplification in colon cancer on EtoBox?
HER 2/neu expression and gene amplification in colon cancer by Daniel R. Nathanson; Alfred T. Culliford IV; Jinru Shia; Beiyun Chen; Matthew D'Alessio; Zhao-Shi Zeng; Garrett M. Nash; William Gerald; Francis Barany; Philip B. Paty is a Medicine article available to read on EtoBox.
What is HER 2/neu expression and gene amplification in colon cancer about?
## Abstract HER 2/__neu__ is an important oncogene in breast cancer, but the prevalence and significance of HER 2/__neu__ gene amplification in colon cancer have been poorly documented. We have evaluated HER 2/__neu__ gene amplification and protein overexpression in a series of colon cancers to assess the frequency, concordance and clinical significance of these events. HER 2/__neu__ gene copy number was measured in 154 primary colon tumors, 15 liver metastases and matched normal tissues using a quantitative PCR/ligase detection reaction (LDR) technique developed and validated in our laboratory. HER 2/__neu__ copy number was confirmed by fluorescent __in situ__ hybridization (FISH) in all tumors found to have gene amplification. In an independent and blinded fashion, HER 2/__neu__ expression was assessed in paraffin sections from 139 of the tumor specimens using the HercepTest kit. HER 2/__neu__ gene amplification was observed in 4 (2.4%) of the 169 tumor specimens and in none of the normal tissues. There was no apparent association with stage of disease, tumor grade or patient survival. Among 139 cases evaluated by immunohistochemistry (IHC), HER 2/__neu__ overexpression was seen
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- Author
- Daniel R. Nathanson; Alfred T. Culliford IV; Jinru Shia; Beiyun Chen; Matthew D'Alessio; Zhao-Shi Zeng; Garrett M. Nash; William Gerald; Francis Barany; Philip B. Paty
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)