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See the Unseen: Red‐Emissive Carbon Dots for Visualizing the Nucleolar Structures in Two Model Animals and In Vivo Drug Toxicity by Ke‐Fei Xu; Hao‐Ran Jia; Zihao Wang; Hui‐Heng Feng; Ling‐Yi Li; Rufeng Zhang; Samran Durrani; Fengming Lin; Fu‐Gen Wu is a Engineering article available to read on EtoBox.

What is See the Unseen: Red‐Emissive Carbon Dots for Visualizing the Nucleolar Structures in Two Model Animals and In Vivo Drug Toxicity about?

## Abstract Nucleolus, which participates in many crucial cellular activities, is an ideal target for evaluating the state of a cell or an organism. Here, bright red‐emissive carbon dots (termed CPCDs) with excitation‐independent/polarity‐dependent fluorescence emission are synthesized by a one‐step hydrothermal reaction between congo red and __p__‐phenylenediamine. The CPCDs can achieve wash‐free, real‐time, long‐term, and high‐quality nucleolus imaging in live cells, as well as in vivo imaging of two common model animals—zebrafish and __Caenorhabditis elegans__ (__C__. __elegans__). Strikingly, CPCDs realize the nucleolus imaging of organs/flowing blood cells in zebrafish at a cellular level for the first time, and the superb nucleolus imaging of __C__. __elegans__ suggests that the germ cells in the spermatheca probably have no intact nuclei. These previously unachieved imaging results of the cells/tissues/organs may guide the zebrafish‐related studies and benefit the research of __C__. __elegans__ development. More importantly, a novel strategy based on CPCDs for in vivo toxicity evaluation of materials/drugs (e.g., Ag^+^), which can visualize the otherwise unseen injuries in z

Who reads See the Unseen: Red‐Emissive Carbon Dots for Visualizing the Nucleolar Structures in Two Model Animals and In Vivo Drug Toxicity?

Author

Ke‐Fei Xu; Hao‐Ran Jia; Zihao Wang; Hui‐Heng Feng; Ling‐Yi Li; Rufeng Zhang; Samran Durrani; Fengming Lin; Fu‐Gen Wu

Publisher

Wiley

Published

2023

Language

EN

Field

Engineering (Physical Sciences)