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This thesis presents the design and characterization of a novel miniaturized stirred bioreactor system (BioXplorer™) for mammalian cell culture, enabling parallel operation of 4-16 bioreactors. The system demonstrates effective process optimization with comparable growth kinetics and product titres to larger scale bioreactors, while facilitating complex feeding strategies. Key engineering parameters such as power input, mixing time, and mass transfer coefficients have been thoroughly characterized, showcasi

Author

Alessandro

Language

EN