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Properties of group I allergens from grass pollen and their relation to cathepsin B, a member of the C1 family of cysteine proteinases by Kay Grobe; Marco Pöppelmann; Wolf-Meinhard Becker; Arnd Petersen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Properties of group I allergens from grass pollen and their relation to cathepsin B, a member of the C1 family of cysteine proteinases about?

Expansins are a family of proteins that catalyze pH‐dependent long‐term extension of isolated plant cell walls. They are divided into two groups, α and β, the latter consisting of the grass group I pollen allergens and their vegetative homologs. Expansins are suggested to mediate plant cell growth by interfering with either structural proteins or the polysaccharide network in the cell wall. Our group reported papain‐like properties of β‐expansin of Timothy grass (__Phleum pratense__) pollen, Phl p 1, and suggested that cleavage of cell wall structural proteins may be the underlying mechanism of expansin‐mediated wall extension. Here, we report additional data showing that β‐expansins resemble ancient and modern cathepsin B, which is a member of the papain (C1) family of cysteine proteinases. Using the __Pichia pastoris__ expression system, we show that cleavage of inhibitory prosequences from the recombinant allergen is facilitated by its N‐glycosylation and that the truncated, activated allergen shows proteolytic activity, resulting in very low stability of the protein. We also show that deglycosylated, full‐length allergen is not activated efficiently and therefore is relatively

Who reads Properties of group I allergens from grass pollen and their relation to cathepsin B, a member of the C1 family of cysteine proteinases?

Author

Kay Grobe; Marco Pöppelmann; Wolf-Meinhard Becker; Arnd Petersen

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)