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The Identification of Myogenic Cells in Skeletal Muscle, with Emphasis on the Use of Tritiated Thymidine Autoradiography and Desmin Antibodies by MATTHEW J. LAWSON-SMITH; JOHN K. McGEACHIE is a Earth and Planetary Sciences article available to read on EtoBox.

What is The Identification of Myogenic Cells in Skeletal Muscle, with Emphasis on the Use of Tritiated Thymidine Autoradiography and Desmin Antibodies about?

The identification of myogenic precursor cells (mpc) is a key factor in determining the early events in the myogenesis and regeneration of skeletal muscle. Although satellite cells have long been established as the providers of myoblastic cells, very little is really known (apart from their anatomical location in relation to muscle fibres and their ability to migrate) about the precise role of satellite cells in myogenesis. Numerous techniques for labelling mpc have been devised, but none of these has proven to be completely reliable in firmly establishing the origin of myogenic cells. The use of tritiated thymidine to label DNA in proliferating mpc (which are not specifically distinguishable at the time) and the subsequent location of their labelled progeny in myotube nuclei has revealed a great deal of data on the timing of myogenesis, but not about the nature of mpc themselves. DNA synthesis can also be detected by antibodies to the thymidine analogue, bromodeoxyuridine, and also by antibody staining for proliferating nuclear cell antigen. Like tritiated thymidine, these other markers are not specific for muscle but are general markers for DNA synthesis. In situ hybridisation of

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Author

MATTHEW J. LAWSON-SMITH; JOHN K. McGEACHIE

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0021-8782)

Published

1998

Language

EN

Field

Earth and Planetary Sciences (Physical Sciences)