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The experiment aimed to determine the optimal pH level for highest peroxidase enzyme activity. Peroxidase from turnip was reacted with hydrogen peroxide and guaiacol at pH levels of 4.0, 7.0, and 8.0. Initial reaction velocity (IRV) was calculated to assess activity. The pH 7.0 trial produced the highest IRV, supporting the hypothesis that neutral pH provides highest activity by maintaining enzyme structure. While the baseline trial showed highest absorbance readings over time, IRV is a better measure of ac

Author
sarahrey
Language
EN