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Introduction of Impermeable Actin-staining Molecules to Mammalian Cells by Optoporation by Dhakal, Kamal; Black, Bryan; Mohanty, Samarendra is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Introduction of Impermeable Actin-staining Molecules to Mammalian Cells by Optoporation about?

The selective insertion of foreign materials, such as fluorescent markers or plasmids, into living cells has been a challenging problem in cell biology due to the cell membrane's selective permeability. However, it is often necessary that researchers insert such materials into cells for various dynamical and/or drug delivery studies. This problem becomes even more challenging if the study is to be limited to specific cells within a larger population, since other transfection methods, such as viral transfection and lipofection, are not realizable with a high degree of spatial selectivity. Here, we have used a focused femtosecond laser beam to create a small transient hole in the cellular membrane (optoporation) in order to inject nanomolar concentrations of rhodamine phalloidin (an impermeable dye molecule for staining filamentous actin) into targeted living mammalian cells (both HEK and primary cortical neurons). Following optoporation, the dye bound to the intracellular actin network and rise in fluorescence intensity was observed. Theoretical dynamics of the dye's diffusion is discussed, and numerical simulations of diffusion time constants are found to match well with experiment

Who reads Introduction of Impermeable Actin-staining Molecules to Mammalian Cells by Optoporation?

Author

Dhakal, Kamal; Black, Bryan; Mohanty, Samarendra

Publisher

Springer Science and Business Media LLC; London: Nature Publishing Group; [London]: Springer Nature; Nature Publishing Group; Society for Mining, Metallurgy and Exploration Inc.; Research Square; Springer Nature; Island Press (ISSN 2045-2322)

Published

2014

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)