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What is Phosphate Assay Method for Clinical Samples about?

This document provides a method for determining phosphate levels in clinical samples using spectrophotometry. Phosphate ions combine with molybdate in acid solution to form a compound that absorbs light at 340nm. Alternatively, the compound can be reduced to form a blue product measured at 650 or 830nm. The method involves preparing phosphate standards and using them to generate a calibration curve to determine phosphate levels in serum samples. Expected phosphate levels in serum are 2.5-4.5 mg/100ml and in

Author

Daria Vîrtic

Language

EN