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Changes to crystals of Escherichia coliβ-galactosidase during room-temperature/low-temperature cycling and their relation to cryo-annealing by Douglas H. Juers; Jeffrey Lovelace; Henry D. Bellamy; Edward H. Snell; Brian W. Matthews; Gloria E. O. Borgstahl is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Changes to crystals of Escherichia coliβ-galactosidase during room-temperature/low-temperature cycling and their relation to cryo-annealing about?

Flash-cooling of macromolecular crystals often compromises diffraction quality by increasing the mosaicity. In some cases, cycling the crystal between low temperature (LT) and room temperature (RT) can reverse this increase in mosaicity. Previous studies of RT/LT cycling have focused on the quality of the crystal as it was repeatedly returned to the LT state. Here, crystal quality is explored not only at LT but also when the crystal is returned to RT. The domain model is used to extract information about crystal order from reflection profiles measured from crystals of Escherichia coli beta-galactosidase at both temperatures. Despite optimization of the cryocooling protocol, the mosaicity increases by about sixfold with cooling and is anisotropic at both temperatures. The mosaicity increase is the consequence of a decrease in domain volume, an increase in the variation of domain cell dimensions and an increase in the angular spread between domains. Upon rewarming, the mosaicity recovers substantially, including the somewhat surprising recovery of domain volume, but incompletely. Over multiple RT/LT cycles disorder in both states increases, which appears to mainly arise from radiatio

Who reads Changes to crystals of Escherichia coliβ-galactosidase during room-temperature/low-temperature cycling and their relation to cryo-annealing?

Author

Douglas H. Juers; Jeffrey Lovelace; Henry D. Bellamy; Edward H. Snell; Brian W. Matthews; Gloria E. O. Borgstahl

Publisher

International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)