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Co-culture of primary human T cells with leukemia cells to measure regulatory T cell expansion by Marius Külp; Laura Diehl; Halvard Bonig; Rolf Marschalek is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Summary The expansion of regulatory T cells (Tregs) is known to be mediated by cytokines including IL-10 and TGFβ but has additionally been shown to depend on the interaction of the immune receptors ICOSLG and ICOS. Here, we describe a co-culture system which enables quantification of the ability of leukemia cells to induce Treg expansion through secreted cytokines and direct receptor interactions. The protocol is applicable for MHC-matched and -unmatched experiments and allows assessment of Treg expansion without using a mouse model. For complete details on the use and execution of this protocol, please refer to Külp et al. (2022).

Who reads Co-culture of primary human T cells with leukemia cells to measure regulatory T cell expansion?

Author

Marius Külp; Laura Diehl; Halvard Bonig; Rolf Marschalek

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)