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Stabilization of unstable CGC^+^ triplex DNA by single-walled carbon nanotubes under physiological conditions by Yujun Song; Lingyan Feng; Jinsong Ren; Xiaogang Qu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Stabilization of unstable CGC^+^ triplex DNA by single-walled carbon nanotubes under physiological conditions about?

Triplex formation is a promising strategy for realizing artificially controlling of gene expression, reversible assembly of nanomaterials and DNA nanomachine and single-walled nanotubes (SWNTs) have been widely used as gene and drug delivery vector or as ‘building blocks’ in nano-/microelectronic devices. CGC^+^ triplex is not as stable as TAT triplex. The poor stability of CGC^+^ triplex limits its use __in vitro__ and __in vivo__. There is no ligand that has been reported to selectively stabilize CGC^+^ triplets rather than TAT. Here, we report that SWNTs can cause d(CT)•d(AG) duplex disproportionation into triplex d(C^+^T)•d(AG)•d(CT) and single-strand d(AG) under physiological conditions. SWNTs can reduce the stringency of conditions for CGC^+^ triplex formation studied by UV–vis, CD, DNA melting, light scattering and atomic force microscopy. Further studies indicate that electrostatic interaction is crucial for d(CT)•d(AG) repartition into triplex d(C^+^T)•d(AG)•d(CT). Our findings may facilitate utilization of SWNTs–DNA complex in artificially controlling of gene expression, nanomaterials assembly and biosensing.

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Author

Yujun Song; Lingyan Feng; Jinsong Ren; Xiaogang Qu

Publisher

Oxford University Press (OUP)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)