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Immunohistochemical Characterization of the Basement Membrane Epitopes in Bis(2-chloroethyl) Sulfide-induced Toxicity in Mouse Ear Skin by Nancy A. Monteiro-Riviere; Alfred O. Inman; Michael C. Babin; Robert P. Casillas is a Environmental Science article available to read on EtoBox.

What is Immunohistochemical Characterization of the Basement Membrane Epitopes in Bis(2-chloroethyl) Sulfide-induced Toxicity in Mouse Ear Skin about?

Sulfur mustard (bis(2-chloroethyl) sulfide (HD)), a potent cutaneous vesicant and bifunctional alkylating agent, produces significant time-dependent histopathological changes in the skin of the mouse. The right ears of male CD1 mice were exposed topically to 5.0 microl of 195 mM (0.16 mg) HD in dichloromethane and harvested at 6, 12, 18 and 24 h. The left ear control was dosed with 5.0 microl of dichloromethane. In all controls and HD-treated mouse ear, moderate immunofluorescence staining was seen at the epidermal-dermal junction with bullous pemphigoid (BP), epidermolysis bullosa acquisita (EBA) and laminin (Lam), and light staining was observed with bullous pemphigoid 180 (BP180), fibronectin (Fn) and type IV collagen (Coll IV). Mouse anti-human monoclonal antibodies for GB3, L3d and 19-DEJ-1 (Uncein) did not cross-react. In microvesicles, BP, BP180 and Fn showed areas of light focal epidermal staining and homogeneous dermal staining, and EBA, Lam and Coll IV showed moderate dermal staining. Both BP and Fn exhibited weak, inconsistent staining with time. Immunoelectron microscopy (IEM) revealed similar results, with an increase in cell damage from 6 to 24 h, which corresponded t

Who reads Immunohistochemical Characterization of the Basement Membrane Epitopes in Bis(2-chloroethyl) Sulfide-induced Toxicity in Mouse Ear Skin?

Author

Nancy A. Monteiro-Riviere; Alfred O. Inman; Michael C. Babin; Robert P. Casillas

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0260-437X)

Published

1999

Language

EN

Field

Environmental Science (Physical Sciences)