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Determination of tramadol by capillary gas chromatography with flame ionization detection: Application to human and rabbit pharmacokinetic studies by Shung-Tai Ho; Jhi-Joung Wang; Wen-Jinn Liaw; Chiu-Ming Ho; Jih-Heng Li is a Chemistry article available to read on EtoBox.

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A rapid, sensitive, precise and accurate capillary gas chromatographic assay with flame ionization detection was developed for the determination of tramadol in human, rabbit, pig and dog plasma. It is comprised of only a one-step extraction procedure with dichloromethane at pH 11.15 and gas chromatography on a capillary column. The recoveries of tramadol and meperidine (internal standard) were greater than 88%. Calibration graphs were linear over the concentration range 12.5-10 000 ng / ml with a coefficient of variation, both within-day and between-day, of less than 10% at any level. The limit of detection was 8 ng / ml of plasma based on signal-to-noise ratio of 3. Six other clinically used analgesics were investigated to check for potential interferences and their analytical conditions. The specificity of this assay was checked with two major metabolites of tramadol (M : O-demethyltramadol; M : N-demethyltramadol). Tramadol in plasma did not decompose 1 2 significantly at 2208C for 56 days. Pharmacokinetic application with intravenous tramadol in humans and rabbits revealed that tramadol followed a two-compartment open model with one distribution phase and one elimination phase.

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Author

Shung-Tai Ho; Jhi-Joung Wang; Wen-Jinn Liaw; Chiu-Ming Ho; Jih-Heng Li

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1999

Language

EN

Field

Chemistry (Physical Sciences)