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Isolation of photorespiratory mutants from Lotus japonicus deficient in glutamine synthetase by Alicia Orea; Purificación Pajuelo; Eloísa Pajuelo; Concepción Quidiello; José M. Romero; Antonio J. Márquez is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Isolation of photorespiratory mutants from Lotus japonicus deficient in glutamine synthetase about?
A mutagenesis programme using ethyl methanesulphonate (EMS) was carried out on __Lotus japonicus__ (Regel) Larsen cv. Gifu in order to isolate photorespiratory mutants in this model legume. These mutants were able to grow in a CO~2~‐enriched atmosphere [0.7% (v/v) CO~2~] but showed stress symptoms when transferred to air. Among them, three mutants displayed low levels of glutamine synthetase (GS; EC 6.3.1.2) activity in leaves. The mutants accumulated ammonium in leaves upon transfer from 0.7% (v/v) CO~2~ to air. F~1~ plants of back crosses to wild type were viable in air and F~2~ populations segregated 3 : 1 (viable in air : air‐sensitive) indicative of a single Mendelian recessive trait. Complementation tests showed that the three mutants obtained were allelic. Chromatography on DEAE‐Sephacel used to separate the cytosolic and plastidic GS isoenzymes together with immunological data showed that: (1) mutants were specifically affected in the plastidic GS isoform, and (2) in __L. japonicus__ the plastidic GS isoform eluted at lower ionic strength than the cytosolic isoform, contrary to what happens in most plants. The plastidic GS isoform present in roots of wild type __L. japonicu
Who reads Isolation of photorespiratory mutants from Lotus japonicus deficient in glutamine synthetase?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Alicia Orea; Purificación Pajuelo; Eloísa Pajuelo; Concepción Quidiello; José M. Romero; Antonio J. Márquez
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0031-9317)
- Published
- 2002
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)