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Determination of the Degree of Bacterialcontamination of Whole-blood Collections Using Anautomated Microbe-detection System by Dirk De Korte; Jan H. Marcelis; A.M. Soeterboek is a Medicine article available to read on EtoBox.

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**BACKGROUND** : The prevalence of bacterial contamination in whole‐blood collections, either with immediate sampling or sampling after overnight storage as whole blood at 20°C, is determined. **STUDY DESIGN AND METHODS** : Whole blood was collected under blood bank conditions in special five‐bag systems, allowing sampling in a closed system for culture bottles. Samples were taken within 2 hours after collection (Group 1) or after overnight storage of the whole blood at 20°C (Group 2). Culture bottles were incubated for 7 days, and positive samples were entered on agar plates for confirmation and determination. **RESULTS** : In Group 1, 9219 units were tested; 27 units were positive with positive subculture, that is, 0.29 percent with a 95% CI of 0.19 to 0.42 percent. In Group 2, 9038 units were tested; 36 units were positive with positive subculture, that is, 0.39 percent with a 95% CI of 0.28 to 0.55 percent. No significant difference could be found between the two test groups. The majority of bacteria were either __Staphylococcus__ (all coagulase‐negative) or __Propionibacterium__ species. **CONCLUSION:** For a total of 18,257 units, 0.34 percent (CI, 0.25‐0.44) of whole‐blood c

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Author

Dirk De Korte; Jan H. Marcelis; A.M. Soeterboek

Publisher

Elsevier Science; John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Elsevier; Elsevier BV; Wiley (ISSN 0372-1248)

Published

2001

Language

EN

Field

Medicine (Health Sciences)