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Characterisation of Reversed-phase Liquid Chromatography Stationary Phases for the Analysis of Basic Pharmaceuticals: Eluent Properties and Comparison of Empirical Test Methods by R.J.M. Vervoort; E. Ruyter; A.J.J. Debets; H.A. Claessens; C.A. Cramers; G.J. de Jong is a Chemistry article available to read on EtoBox.

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The reversed-phase liquid chromatographic analysis of basic pharmaceuticals can be problematic. Both the properties of the eluent and the stationary phase can influence the chromatographic performance. Therefore selection of suitable experimental conditions for the analysis of basic compounds can be difficult. This paper shows that the organic modifier and the nature of the buffer influence the eluent properties. Moreover, the nature and amount of modifier also influence the basicity of the analytes. Investigations showed that the nature of the buffer can have a significant influence on retention and peak shape of basic compounds. Test procedures using basic analytes as test probes provided relevant information with respect to selecting columns to analyse basic pharmaceutical compounds. Test procedures using compounds like aniline, phenol and benzene were found to be less suitable.

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Author

R.J.M. Vervoort; E. Ruyter; A.J.J. Debets; H.A. Claessens; C.A. Cramers; G.J. de Jong

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2001

Language

EN

Field

Chemistry (Physical Sciences)