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Recto-Anal Junction (RAJ) and Fecal Microbiomes of Cattle Experimentally Challenged With Escherichia coli O157:H7 by Mir, Raies A.; Schaut, Robert G.; Looft, Torey; Allen, Heather K.; Sharma, Vijay K.; Kudva, Indira T. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Recto-Anal Junction (RAJ) and Fecal Microbiomes of Cattle Experimentally Challenged With Escherichia coli O157:H7 about?

Cattle are the asymptomatic reservoirs of __Escherichia coli__ O157:H7 (O157) that preferentially colonizes the bovine recto-anal junction (RAJ). Understanding the influence of O157 on the diversity of the RAJ microbiota could give insights into its persistence at the RAJ in cattle. Hence, we compared changes in bovine RAJ and fecal microbiota following O157 challenge under experimental conditions. Cattle were either orally challenged (__n__ = 4) with10^10^ CFU of a streptomycin-resistant O157 strain 86–24, or mock-challenged (__n__ = 4) with phosphate buffered saline. Rectoanal mucosal swab (RAMS) and fecal samples were collected at different time points for analysis. Alpha diversity measures (Chao1 species richness and Shannon diversity index) were found to be significantly different between RAMS and fecal samples but not influenced by O157 challenge. The __Firmicutes__ to __Bacteroidetes__ (F: B) ratio was higher in RAMS samples from O157 colonized animals and this may have influenced the consistent yet decreased O157 colonization at the RAJ. Specific bacterial genera that were present in relative low abundance in fecal and RAMS microbiota did not affect overall microbial divers

Who reads Recto-Anal Junction (RAJ) and Fecal Microbiomes of Cattle Experimentally Challenged With Escherichia coli O157:H7?

Author

Mir, Raies A.; Schaut, Robert G.; Looft, Torey; Allen, Heather K.; Sharma, Vijay K.; Kudva, Indira T.

Publisher

Frontiers; Frontiers Media SA; Frontiers Media S.A.; Lausanne: Frontiers Media S.A., 2010-; Publons (ISSN 1664-302X)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)