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Can I read Interpreting the Difference Between Conventional and Bi-directional Plate-height Measurements in Liquid Chromatography on EtoBox?
Interpreting the Difference Between Conventional and Bi-directional Plate-height Measurements in Liquid Chromatography by Hui Guo; Douglas D. Frey is a Chemistry article available to read on EtoBox.
What is Interpreting the Difference Between Conventional and Bi-directional Plate-height Measurements in Liquid Chromatography about?
An experimental and theoretical study was conducted of the column characterization technique in which plate heights determined using the conventional pulse-response method are compared with those determined using a bi-directional method where an eluite sample is introduced into one end of a chromatographic column and elution occurs at the same end after the flow direction is reversed inside the column. Experiments are presented for a micropellicular HPLC column before and after its performance has been degraded by repeated sample injections, for a low-pressure column containing nonporous glass particles, and for an HPLC column containing particles with 300 Å pores. The results obtained are interpreted in terms of several different theories which apply in various Fourier number ranges. It was shown that the transcolumn contribution to convective dispersion in a chromatographic column is largely responsible for the difference observed between conventional and bi-directional plate-height measurements and that a collocation method can be employed to develop a useful analytical expression for this contribution.
Who reads Interpreting the Difference Between Conventional and Bi-directional Plate-height Measurements in Liquid Chromatography?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Hui Guo; Douglas D. Frey
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2010
- Language
- EN
- Field
- Chemistry (Physical Sciences)