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TBP-interacting protein TIP120A is a new global transcription activator with bipartite functional domains by Kentaro Kayukawa; Yoko Kitajima; Taka-aki Tamura is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background We previously identified a TBP (TATA‐binding protein)‐interacting protein 120A (TIP120A) from rat liver nuclear extracts. TIP120A is thought to be a unique global transcription factor that can interact with TBP and can stimulate all classes of eukaryotic transcription. ## Results We produced various truncation proteins of TIP120A to delineate its functional domains. TIP120A binds to TBP in the acidic amino acid‐rich N‐terminal region and in the leucine‐rich C‐terminal region. These regions exhibited an ability to stimulate basal transcription __in vitro__. In addition, these two regions overlap with domains that facilitate nonspecific DNA‐binding of RNA polymerase II. The sequences of these two regions are significantly conserved among TIP120A homologues of eukaryotes. ## Conclusions TIP120A is a bipartite transcription factor, and both N‐terminal and C‐terminal regions exhibit TBP‐binding activity and stimulate the basal transcription ability.

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Author

Kentaro Kayukawa; Yoko Kitajima; Taka-aki Tamura

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1356-9597)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)