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Stimulation of Adenylyl Cyclase Mediated by Phospholipase C‐Linked M~3~ Muscarinic Receptor in Human Neuroblastoma SK‐N‐BE(2)C Cells by Byung-Chang Suh; Kyong-Tai Kim is a Neuroscience article available to read on EtoBox.
What is Stimulation of Adenylyl Cyclase Mediated by Phospholipase C‐Linked M~3~ Muscarinic Receptor in Human Neuroblastoma SK‐N‐BE(2)C Cells about?
Abstract: Muscarinic receptor in human neuroblastoma SK‐N‐BE(2)C cells was identified and characterized. Treatment of the cells with carbachol evoked the generation of inositol 1,4,5‐trisphosphate (IP~3~) with a peak level reached at 1 min after stimulation. Carbachol increased intracellular Ca^2+^ ([Ca^2+^]~i~) with an EC~50~ value of 35 μ__M__. In addition, carbachol produced a 1.3–3‐fold increase in the cyclic AMP (cAMP) level compared with untreated control and elevated synergistically the cAMP level in the treatment with prostaglandin E~2~ (PGE~2~). The M~3~ antagonist __p__‐fluorohexahydrosiladifenidol (IC~50~ = 0.5–0.8 μ__M__) inhibited the increases in [Ca^2+^]~i~, IP~3~, and cAMP more effectively than the M~1~ antagonist pirenzepine (IC~50~ = 5–9 μ__M__) and the M~2~ antagonist methoctramine (IC~50~ = 20–30 μ__M__). The involvements of [Ca^2+^]~i~ elevation and protein kinase C activation induced by phospholipase C activation were tested in the carbachol‐induced cAMP production. The calcium chelator BAPTA/AM (75 μ__M__) inhibited significantly the synergistic effects of carbachol and PGE~2~ on the production of cAMP, whereas the Ca^2+^ ionophore ionomycin (1 μ__M__) clearl
Who reads Stimulation of Adenylyl Cyclase Mediated by Phospholipase C‐Linked M~3~ Muscarinic Receptor in Human Neuroblastoma SK‐N‐BE(2)C Cells?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Byung-Chang Suh; Kyong-Tai Kim
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 1995
- Language
- EN
- Field
- Neuroscience (Life Sciences)