Can I read Attenuation of enoyl coenzyme A hydratase short chain 1 expression in gastric cancer cells inhibits cell proliferation and migration in vitro on EtoBox?

Attenuation of enoyl coenzyme A hydratase short chain 1 expression in gastric cancer cells inhibits cell proliferation and migration in vitro by Xiao-San Zhu; Peng Gao; Yi-Chen Dai; Jun-Pei Xie; Wei Zeng; Qing-Na Lian is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Attenuation of enoyl coenzyme A hydratase short chain 1 expression in gastric cancer cells inhibits cell proliferation and migration in vitro about?

## Abstract Enoyl coenzyme A hydratase short chain 1 (ECHS1) is an important part of the mitochondrial fatty acid β-oxidation pathway. Altered ECHS1 expression has been implicated in cancer cell proliferation. This study assessed ECHS1 expression in human gastric cancer cell lines and investigated the effects of ECHS1 knockdown on gastric cancer cell proliferation and migration. The human gastric cancer cell lines SGC-7901, BGC-823 and MKN-28, and the immortalized human gastric epithelial mucosa GES-1 cell line were analyzed for ECHS1 protein levels using western blot. The effectiveness of ECHS1-RNA interference was also determined using western blot. Proliferation and migration of the siECHS1 cells were respectively measured with the CCK-8 and transwell assays. Phosphorylation of PKB and GSK3β was assessed using western blot. ECHS1 protein levels were significantly higher in poorly differentiated cells than in well-differentiated cells and immortalized gastric epithelial mucosa cells. Stable expression of ECHS1 shRNA was associated with an over 41% reduction in the ECHS1 protein levels of siECHS1 cells. Constitutive knockdown of the ECHS1 gene in siECHS1 cells was associated with

Who reads Attenuation of enoyl coenzyme A hydratase short chain 1 expression in gastric cancer cells inhibits cell proliferation and migration in vitro?

Author

Xiao-San Zhu; Peng Gao; Yi-Chen Dai; Jun-Pei Xie; Wei Zeng; Qing-Na Lian

Publisher

SP Versita; Springer (Biomed Central Ltd.); BioMed Central Ltd.; Springer Science and Business Media LLC; Walter de Gruyter GmbH (ISSN 1425-8153)

Published

2014

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)