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Can I read Quantitative DNA hybridization in solution using magnetic/luminescent core–shell nanoparticles on EtoBox?
Quantitative DNA hybridization in solution using magnetic/luminescent core–shell nanoparticles by Ahjeong Son; Dosi Dosev; Mikaela Nichkova; Zhiya Ma; Ian M. Kennedy; Kate M. Scow; Krassimira R. Hristova is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Quantitative DNA hybridization in solution using magnetic/luminescent core–shell nanoparticles about?
Nanoscale magnetic/luminescent core-shell particles were used for DNA quantification in a hybridization-in-solution approach. We demonstrated a rapid, simple, and non-polymerase chain reaction-based DNA hybridization-in-solution assay for quantifying bacteria capable of biodegrading methyl tertiary-butyl ether. Fe 3 O 4 /Eu:Gd 2 O 3 core-shell nanoparticles synthesized by spray pyrolysis were biofunctionalized with NeutrAvidin. Following immobilization of a biotinylated probe DNA on the particles' surfaces via passive adsorption, target DNA labeled with fluorescein isothiocyanate was hybridized with probe DNA. The hybridized DNA complex was separated from solution with a magnet, while nonhybridized DNA remained in solution. The normalized fluorescence (fluorescein isothiocyanate/ nanoparticles) measured with a spectrofluorometer indicated a linear quantification (R 2 = 0.98) of the target bacterial 16 S rDNA. The rate of hybridization increased concurrently with the target DNA concentration. In addition, this approach differentiated between the signal outputs from perfectly complementary target and two-base mismatched target DNA in a range of concentrations, showing the specificity
Who reads Quantitative DNA hybridization in solution using magnetic/luminescent core–shell nanoparticles?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ahjeong Son; Dosi Dosev; Mikaela Nichkova; Zhiya Ma; Ian M. Kennedy; Kate M. Scow; Krassimira R. Hristova
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)