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Pyruvate protection against β‐amyloid‐induced neuronal death: Role of mitochondrial redox state by Gema Alvarez; Milagros Ramos; Francisca Ruiz; Jorgina Satrústegui; Elena Bogónez is a Neuroscience article available to read on EtoBox.

What is Pyruvate protection against β‐amyloid‐induced neuronal death: Role of mitochondrial redox state about?

## Abstract The mechanism by which β‐amyloid protein (Aβ) causes degeneration in cultured neurons is not completely understood, but several lines of evidence suggest that Aβ‐mediated neuronal death is associated with an enhanced production of reactive oxygen species (ROS) and oxidative damage. In the present study, we address whether supplementation of glucose‐containing culture media with energy substrates, pyruvate plus malate (P/M), protects rat primary neurons from Aβ‐induced degeneration and death. We found that P/M addition attenuated cell death evoked by β‐amyloid peptides (Aβ~25–35~ and Aβ~1–40~) after 24 hr treatment and that this effect was blocked by α‐ciano‐3‐hydroxycinnamate (CIN), suggesting that it requires mitochondrial pyruvate uptake. P/M supply to control and Aβ‐treated neuronal cultures increases cellular reducing power, as indicated by the ability to reduce the dye 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT). The early increases in ROS levels, measured by dichlorofluorescein (DCF) fluorescence, and caspase‐3 activity that follow exposure to Aβ were notably reduced in the presence of P/M. These results place activation of caspase‐3 most li

Who reads Pyruvate protection against β‐amyloid‐induced neuronal death: Role of mitochondrial redox state?

Author

Gema Alvarez; Milagros Ramos; Francisca Ruiz; Jorgina Satrústegui; Elena Bogónez

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

2003

Language

EN

Field

Neuroscience (Life Sciences)