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Yolk-shell nanospheres based silane-functionalized carbon dots: Designed synthesis and application in highly selective determination of aflatoxin biosynthesis-related genes nor-1 in cereal crops by Yaqi Li; Tao Yu; Shuangfeng Peng; Qingyue Sun; Dezhao Kong; Chang Liu; Qi Zhang; Qiaoqiao Shi; Yong Chen is a Chemistry article available to read on EtoBox.
What is Yolk-shell nanospheres based silane-functionalized carbon dots: Designed synthesis and application in highly selective determination of aflatoxin biosynthesis-related genes nor-1 in cereal crops about?
This study presented advanced monitoring approaches for the aflatoxin gene cluster in Aspergillus flavus and Aspergillus parasiticus, which are effective tools for controlling aflatoxin proliferation in the food chain. A novel approach involved a ratiometric fluorescence probe based on yolk-shell nanospheres (bCDs@SiO 2 @gQDs). The probe's fluorescence was activated by DNA hybridization with the modified bCDs@SiO 2 @gQDs and gold nanoparticles (AuNPs), functioning through donor-acceptor pairs. The exceptional properties of the gQDs and the high FRET efficiency of this system established a functionalized platform for DNA labeling. When the nor-1 gene was present, FRET between the gQDs and AuNPs triggered fluorescence quenching, enabling nor-1 gene detection. The yolk-shell nanostructure of the fluorescent nanoprobes provided an internal reference, reducing experimental errors due to background noise, instrumentation factors, and probe concentration. This allowed for accurate and highly sensitive quantitative determination of the nor-1 gene. The ratiometric fluorescence assay demonstrated a broad linear range (0.1-100 nM) and a low detection limit (12.83 pM) for the nor-1 gene. Tests
Who reads Yolk-shell nanospheres based silane-functionalized carbon dots: Designed synthesis and application in highly selective determination of aflatoxin biosynthesis-related genes nor-1 in cereal crops?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Yaqi Li; Tao Yu; Shuangfeng Peng; Qingyue Sun; Dezhao Kong; Chang Liu; Qi Zhang; Qiaoqiao Shi; Yong Chen
- Publisher
- Elsevier BV
- Published
- 2024
- Language
- EN
- Field
- Chemistry (Physical Sciences)