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Clonal Origin of Trisomy for Chromosome 7 in the Epithelial Compartment of Colon Neoplasia by Jos Herbergs; Jan-Willem Arends; Ernie M.H. F. Bongers; Frans C. S. Ramaekers; Anton H. N. Hopman is a Medicine article available to read on EtoBox.

What is Clonal Origin of Trisomy for Chromosome 7 in the Epithelial Compartment of Colon Neoplasia about?

In this study, we demonstrated the clonal origin of trisomy for chromosome 7 in epithelial cells of colon neoplasia. By using the double-target fluorescence in situ hybridization (FISH) technique in frozen tissue sections that were also immunootained for keratin and vimentin, ratio analysis of FISH signals for chromosomes 7 and I 7 could be performed in epithelial (cytokeratinpositive) or stromal (vimentin-positive) areas. The data demonstrated that trisomy for chromosome 7 is found exclusively in the epithelial compartments and not in the stroma of colon ademarcinoma. We then demonstrated the Occurrence of trisomy for chromosome 7 in the different types of epithelial neoplastic cells, i.e., columnar and goblet cells, which were isolated from frozen tissue sections by mechanical disaggregation of colon tissue and mild l p i s of the cells while protease activity was inhibited. In these cell suspensions, the columnar cells were detected with an antibody to villin, and the goblet cells were stained for mucin, whereas all cells were subsequently subjected t o FISH for chromosome 7. For analysis of neuroendocrine cells, which are present in a very low frequency in colon neoplasia, froz

Who reads Clonal Origin of Trisomy for Chromosome 7 in the Epithelial Compartment of Colon Neoplasia?

Author

Jos Herbergs; Jan-Willem Arends; Ernie M.H. F. Bongers; Frans C. S. Ramaekers; Anton H. N. Hopman

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 1045-2257)

Published

1996

Language

EN

Field

Medicine (Life Sciences)