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3D Dissection of Structural Membrane-Wall Contacts in Filamentous Moss Protonemata by Dominik Harant; Ingeborg Lang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is 3D Dissection of Structural Membrane-Wall Contacts in Filamentous Moss Protonemata about?

In conventional light microscopy, the adjacent cell walls of filamentous moss protonemata are seen from its narrow side thereby obscuring the major area of cell-cell connection. Optical sectioning, segmentation and 3D reconstructions allow the tilting and rotation of intracellular structures thereby greatly improving our understanding of interaction between organelles, membranes and the cell wall. Often, the findings also allow for conclusions on the respective functions. The moss Physcomitrium (Physcomitrella) patens is a model organism for growth, development and morphogenesis. Its filamentous protonemata are ideal objects for microscopy. Here, we investigated the cell wall between two neighboring cells and the connection of membranes towards this wall after plasmolysis in 0.8 M mannitol. An m-green fluorescent protein (GFP)-HDEL cell line was used to visualize the endoplasmatic reticulum (ER), the plasma membrane (PM) was stained with FM4-64. Our studies clearly show the importance of cell-cell contacts in P. patens protonemata. In 86% of the investigated cell pairs, at least one of the protoplasts remained fully attached to the adjacent cell wall. By tilting of z-stacks, volume

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Author

Dominik Harant; Ingeborg Lang

Publisher

MDPI AG

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)