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Electrochemical measurements of glucose dehydrogenase activity exhibited by Escherichia coli cells; effects of the additions of pyrroloquinoline quinone, magnesium or calcium ions and ethylenediaminetetraacetic acid by Dyah Iswantini; Kan Kato; Kenji Kano; Tokuji Ikeda is a Engineering article available to read on EtoBox.
What is Electrochemical measurements of glucose dehydrogenase activity exhibited by Escherichia coli cells; effects of the additions of pyrroloquinoline quinone, magnesium or calcium ions and ethylenediaminetetraacetic acid about?
An electrode modified with immobilized Escherichia coli K12 cells produces a steady-state catalytic current originating from the Ž . glucose dehydrogenase activity GDH of the bacterial cells. Thus, the catalytic current provides a convenient means of measuring the bacterial GDH activity. E. coli K12 cells cultivated under ordinary conditions exhibit a GDH activity, the magnitude of which is about Ž . 15% of the full activity attained in the presence of pyrroloquinoline quinone PQQ and magnesium ions. The E. coli K12 cells mostly lose the full GDH activity when treated with ethylenediaminetetraacetic acid, and restore the activity by the additions of PQQ and magnesium or calcium ions. The rate and degree of the restoration of GDH activity strongly depend on the concentrations of PQQ and the divalent cations. The concentration dependence of the bacterial GDH activity is similar to that of the catalytic activity of purified apo-GDH except that the bacterial GDH activity is fully restored by calcium ion as well as magnesium ion, while catalytic activity of apo-GDH is only partially restored by calcium ion.
Who reads Electrochemical measurements of glucose dehydrogenase activity exhibited by Escherichia coli cells; effects of the additions of pyrroloquinoline quinone, magnesium or calcium ions and ethylenediaminetetraacetic acid?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Dyah Iswantini; Kan Kato; Kenji Kano; Tokuji Ikeda
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1567-5394)
- Published
- 1998
- Language
- EN
- Field
- Engineering (Physical Sciences)
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