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N-octadecyl lactose-amide modified microemulsions as targeting delivery carrier for α-linolenic acid: In vitro evaluation and interaction mechanism 1 1 Introduction 1 2 Materials and methods 2 2.1 Reagents 2 2.2 Synthesis and characterization of N-octadecyl lactose-amide 2 2.2.1 Synthesis of N-octadecyl lactose-amide (NOL) 2 2.2.2 Characterization of NOL 3 2.2.2.1 Fourier transform infrared spectroscopy. 3 2.2.2.2 1H NMR and13CNMR measurements 3 2.3 Preparation of MEs 3 2.3.1 Preparation of ALA-loaded MEs modified with NOL 3 2.4 Characterization of MEs 3 2.4.1 Particle size distribution and polydispersity index 3 2.4.2 The pH and electrical conductivity (EC) 3 2.4.3 The FT-IR assessment 3 2.4.4 Morphology observation 3 2.5 Antioxidant assays 3 2.6 In vitro release determination 3 2.7 Biological assessment 3 2.7.1 Cell culture 3 2.7.2 Cell viability assay 3 2.7.3 Determination of cell morphology 4 2.7.4 LDH assay 4 2.8 Interaction mechanism by molecular docking 4 2.9 Statistical analysis 4 3 Results and discussion 4 3.1 Synthesis and identification of NOL 4 3.2 Characterization of ALA ME modified by NOL 4 3.3 Antioxidant stability test 6 3.4 In vitro release of MEs 6 3.5 Biological

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nonfiction